Kjeldahl determination of protein content in Robinia pseudoacacia

The Robinia plant uses flowers as medicine, and the medicinal material is called Robinia pseudoacacia. Robinia pseudoacacia contains abundant proteins, sugars, amino acids, enzymes and many trace elements. Hedgehog is not only widely distributed, but also widely used. Not only medicinal but also bougainvillea can be eaten. It has now become a green vegetable in the city. The KDN-04C Kjeldahl nitrogen analyzer was used to determine the protein content of 10 different sources of Robinia pseudoacacia, which provided the basis for quality evaluation and resource development and utilization.

Proteins are nitrogen-containing organic compounds. The nitrogen-containing organic compounds in the Robinia pseudoacacia flower, together with the sulfuric acid and the catalyst, are heated and digested by the KDN-04C Kjeldahl apparatus to decompose the protein, and the decomposed ammonia is combined with the sulfuric acid to produce ammonium sulfate. Then alkalized distillation to ammonia free, absorbed with boric acid and then titrated with hydrochloric acid standard solution, acid consumption multiplied by the conversion factor, which is the protein content.

The Robinia pseudoacacia flower was derived from the dried flowers and buds of Robinia pseudoacacia. The Kjeldahl instrument was used to determine the protein of the Robinia pseudoacacia flower and flower buds from different habitats. Through experiments, the highest protein content of Robinia pseudoacacia in different production areas of Liaoning Province was 29.07% in Panjin, and the lowest was 25.73% in Dandong Donggang with an average content of 27.23%; the highest protein content in Robinia pseudoacacia flower bud was 34.55% in Wafangdian and the lowest in Dalian. Daheishan 28.85%, with an average content of 31.52%. The value of the KDN-04C Kjeldahl analyzer can be concluded that the average protein content in the flower buds of Robinia pseudoacacia is higher than that of about 2.67% in Robinia pseudoacacia flower.

Weigh the relevant sample in the test when pour Kjeldahl instrument should not be stuck to the bottle wall, this is to avoid the sample in the digestive juice after the sample is not digested thoroughly will make the instrument to determine The result is low, so before this should be first flushed into the bottom of the bottle with water, which is one of the important considerations in the KDN-04C Kjeldahl test.

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